rabbit polyclonal atf3 antibody  (Abmart Inc)

Journal: Frontiers in Cell and Developmental Biology

Article Title: DA. Vra1 -congenic rats display increased gene expression and Schwann cell apoptosis but unaffected nerve regeneration compared to parental DA rats after sciatic nerve injury and repair

doi: 10.3389/fcell.2025.1536347

Figure Lengend Snippet: Boxplots of the axonal outgrowth (A) , total DAPI-stained cells (B) , expression of activating transcription factor 3 (ATF3; (C, D) ), cleaved caspase 3 (E, F) , 27-kDa heat shock protein (HSP27; i.e., Hspb1; (G, H) ) in DA and DA. Vra1 rats 6 days after sciatic nerve transection injury and immediate nerve repair. Values are based on the immunohistochemistry of sciatic nerves at the site of the lesion (SNL; (C, E, G) ) and in the distal nerve ends (SND; (D, F, H) ). The box plots represent the median (line) with 25th and 75th percentiles (Tukey’s hinge) as well as min–max (outliers are marked as o).

Article Snippet: Activated and apoptotic Schwann cells were stained by rabbit anti-ATF3 polyclonal antibody (1:200; Santa Cruz Biotechnology, United States) and anti-cleaved caspase 3 antibody (1:200; BioNordika, Stockholm, Sweden), both diluted in 0.25% Triton-X 100% and 0.25% BSA in PBS.

Techniques: Staining, Expressing, Immunohistochemistry

Journal: Frontiers in Cell and Developmental Biology

Article Title: DA. Vra1 -congenic rats display increased gene expression and Schwann cell apoptosis but unaffected nerve regeneration compared to parental DA rats after sciatic nerve injury and repair

doi: 10.3389/fcell.2025.1536347

Figure Lengend Snippet: Immunohistochemical staining at the site of the lesion (SNL) of transected and repaired sciatic nerves from DA (A, C, E, G, I) and DA. Vra1 congenic (B, D, F, H, J) strain rats, showing staining for ATF3 (A, B) , double staining of ATF3/S-100 (C, D) , cleaved caspase 3 (E, F) , double staining cleaved caspase 3 and S-100 (G, H) as well as HSP27 (i.e., Hspb1) (I, J) . Bar = 100 µm.

Article Snippet: Activated and apoptotic Schwann cells were stained by rabbit anti-ATF3 polyclonal antibody (1:200; Santa Cruz Biotechnology, United States) and anti-cleaved caspase 3 antibody (1:200; BioNordika, Stockholm, Sweden), both diluted in 0.25% Triton-X 100% and 0.25% BSA in PBS.

Techniques: Immunohistochemical staining, Staining, Double Staining

Journal: Frontiers in Cell and Developmental Biology

Article Title: DA. Vra1 -congenic rats display increased gene expression and Schwann cell apoptosis but unaffected nerve regeneration compared to parental DA rats after sciatic nerve injury and repair

doi: 10.3389/fcell.2025.1536347

Figure Lengend Snippet: Boxplots of expression of activating transcription factor 3 (ATF3; (A) ) and 27-kDa heat shock protein (HSP27; i.e., Hspb1; (B) ) in dorsal root ganglia (DRG) sensory neurons from DA and DA. Vra1 rats 6 days after a sciatic nerve transection injury and immediate nerve repair. Values are based on the immunohistochemistry of DRG from the uninjured and injured sides. A ratio for HSP27 (injured/uninjured) is presented in (C) . The box plots represent the median (line) with 25th and 75th percentiles (Tukey’s hinge) as well as min–max (outliers are marked as o).

Article Snippet: Activated and apoptotic Schwann cells were stained by rabbit anti-ATF3 polyclonal antibody (1:200; Santa Cruz Biotechnology, United States) and anti-cleaved caspase 3 antibody (1:200; BioNordika, Stockholm, Sweden), both diluted in 0.25% Triton-X 100% and 0.25% BSA in PBS.

Techniques: Expressing, Immunohistochemistry

Journal: Frontiers in Cell and Developmental Biology

Article Title: DA. Vra1 -congenic rats display increased gene expression and Schwann cell apoptosis but unaffected nerve regeneration compared to parental DA rats after sciatic nerve injury and repair

doi: 10.3389/fcell.2025.1536347

Figure Lengend Snippet: Images of immunohistochemical staining of dorsal root ganglia (DRG) on the injured side where the sciatic nerve was transected and repaired in DA (A, C) and DA. Vra1 congenic (B, D) strain rats demonstrating staining for ATF3 (A, B) and HSP27 (i.e., Hspb1) (C, D) ; the latter was analyzed in both sensory neurons and in satellite cells. Bar = 200 µm.

Article Snippet: Activated and apoptotic Schwann cells were stained by rabbit anti-ATF3 polyclonal antibody (1:200; Santa Cruz Biotechnology, United States) and anti-cleaved caspase 3 antibody (1:200; BioNordika, Stockholm, Sweden), both diluted in 0.25% Triton-X 100% and 0.25% BSA in PBS.

Techniques: Immunohistochemical staining, Staining

Journal: Frontiers in Cell and Developmental Biology

Article Title: DA. Vra1 -congenic rats display increased gene expression and Schwann cell apoptosis but unaffected nerve regeneration compared to parental DA rats after sciatic nerve injury and repair

doi: 10.3389/fcell.2025.1536347

Figure Lengend Snippet: HSP27 (A, D) and ATF3 (B, E) immunoreactivity in part of a dorsal root ganglion on the injured side, where the sciatic nerve was transected and repaired in DA (A, B, C) and DA. Vra1 (D, E, F) rats. Merged images are shown in (C, F) . The arrows mark neurons with different sizes in the different images. Bar = 50 μm.

Article Snippet: Activated and apoptotic Schwann cells were stained by rabbit anti-ATF3 polyclonal antibody (1:200; Santa Cruz Biotechnology, United States) and anti-cleaved caspase 3 antibody (1:200; BioNordika, Stockholm, Sweden), both diluted in 0.25% Triton-X 100% and 0.25% BSA in PBS.

Techniques:

Journal: Frontiers in Cell and Developmental Biology

Article Title: DA. Vra1 -congenic rats display increased gene expression and Schwann cell apoptosis but unaffected nerve regeneration compared to parental DA rats after sciatic nerve injury and repair

doi: 10.3389/fcell.2025.1536347

Figure Lengend Snippet: Scatter diagram from correlation analysis in DA rats (A, C) and DA.Vra1 rats (B, D) showing correlations between ATF3 (%; (A, B) ) and HSP27 (27-kDa heat shock protein; i.e., Hspb1; %; (C, D) ) at the proximal part of the nerve (SNL) and axonal outgrowth (µm).

Article Snippet: Activated and apoptotic Schwann cells were stained by rabbit anti-ATF3 polyclonal antibody (1:200; Santa Cruz Biotechnology, United States) and anti-cleaved caspase 3 antibody (1:200; BioNordika, Stockholm, Sweden), both diluted in 0.25% Triton-X 100% and 0.25% BSA in PBS.

Techniques:

Journal: Frontiers in Cell and Developmental Biology

Article Title: DA. Vra1 -congenic rats display increased gene expression and Schwann cell apoptosis but unaffected nerve regeneration compared to parental DA rats after sciatic nerve injury and repair

doi: 10.3389/fcell.2025.1536347

Figure Lengend Snippet: Boxplots of the relative gene expression of Atf3 (A) , Jun (B) , Casp 3 (C) , Hspb1 (D) , Gsta4 (E) and Nrf2 (F) in the uninjured and injured sciatic nerves in DA and DA. Vra1 congenic rat strains. The box plots represent the median (line) with 25th and 75th percentiles (Tukey’s hinge) as well as min-max (outliers are marked as o).

Article Snippet: Activated and apoptotic Schwann cells were stained by rabbit anti-ATF3 polyclonal antibody (1:200; Santa Cruz Biotechnology, United States) and anti-cleaved caspase 3 antibody (1:200; BioNordika, Stockholm, Sweden), both diluted in 0.25% Triton-X 100% and 0.25% BSA in PBS.

Techniques: Gene Expression

Journal: Frontiers in Cell and Developmental Biology

Article Title: DA. Vra1 -congenic rats display increased gene expression and Schwann cell apoptosis but unaffected nerve regeneration compared to parental DA rats after sciatic nerve injury and repair

doi: 10.3389/fcell.2025.1536347

Figure Lengend Snippet: Boxplots of the relative gene expression of Atf3 (A) , Jun (B) , Hspb1 (C) , Gsta4 (D) and Nrf2 (E) in DRG on the uninjured and injured sides in DA and DA. Vra1 congenic rat strains. The box plots represent the median (line) with 25th and 75th percentiles (Tukey’s hinge) as well as min–max (outliers–marked as o).

Article Snippet: Activated and apoptotic Schwann cells were stained by rabbit anti-ATF3 polyclonal antibody (1:200; Santa Cruz Biotechnology, United States) and anti-cleaved caspase 3 antibody (1:200; BioNordika, Stockholm, Sweden), both diluted in 0.25% Triton-X 100% and 0.25% BSA in PBS.

Techniques: Gene Expression

Journal: Science Advances

Article Title: Sensory-motor circuit is a therapeutic target for dystonia musculorum mice, a model of hereditary sensory and autonomic neuropathy 6

doi: 10.1126/sciadv.adj9335

Figure Lengend Snippet: ( A ) In Dst GT and Wnt1-Cre ; Dst cGT mice, ATF3 was expressed in some DRG neurons together with neurofilament (NF) accumulation (arrowheads) at 3 to 4 weeks of age. Increases in ATF3 and NF were rarely observed in Wnt1-Cre ; Dst cRescue mice. The dotted line indicates the edge of the DRG. ( B ) VGluT1-positive axon terminals were observed in the lumbar SC. In the anterior horn (AH), ChAT-positive motor neurons were surrounded by VGluT1-positive axon terminals. There were fewer VGluT1-positive axon terminals in the AH of Dst GT and Wnt1-Cre ; Dst cGT mice than in Ctrl mice, but they were normally distributed in Wnt1-Cre ; Dst cRescue mice. The dotted line indicates the edge of the SC. ( C ) Quantitative data showed a significant increase in numbers of ATF3-positive cells and NF-accumulating cells in Dst GT and Wnt1-Cre ; Dst cGT mice [ATF3, Ctrl ( n = 5 mice); Wnt1-Cre ; Dst cGT ( n = 3); Dst GT ( n = 5); Wnt1-Cre ; Dst cRescue ( n = 6); NF, Ctrl ( n = 6); Wnt1-Cre ; Dst cGT ( n = 3); Dst GT ( n = 4); Wnt1-Cre ; Dst cRescue ( n = 3)]. Quantitative data showed a significant decrease in number of VGluT1-positive axon terminals in the AH of Wnt1-Cre ; Dst cGT mice. VGluT1-positive axon terminals were significantly increased in Wnt1-Cre ; Dst cRescue mice than Dst GT mice [Ctrl ( n = 12); Wnt1-Cre ; Dst cGT ( n = 3); Dst GT ( n = 6); Wnt1-Cre ; Dst cRescue ( n = 5)]. The number of ChAT-positive cells was statistically same between groups [Ctrl ( n = 8); Wnt1-Cre ; Dst cGT ( n = 3); Dst GT ( n = 5); Wnt1-Cre ; Dst cRescue ( n = 5)]. Scale bars, 50 μm (A) and 200 μm (B). Data are presented as mean ± SE. P > 0.05 (ns), * P < 0.05, ** P < 0.01, and *** P < 0.005, using ANOVA with Tukey’s test in (C).

Article Snippet: For IHC, deparaffinized sections were treated with microwave irradiation in 10 mM citric acid buffer (pH 6.0) for 5 min and incubated overnight at 4°C with the following primary antibodies: rabbit polyclonal anti-ATF3 antibody (1:2000; Santa Cruz Biotechnology; sc-188), rabbit polyclonal anti-VGluT1 antibody (1:1000; Frontier Institute, Hokkaido, Japan; Rb-Af500), goat polyclonal anti-VGluT1 antibody (1:1000; Frontier Institute; Go-Af310), goat polyclonal anti-ChAT antibody (1:500; Millipore; AB144P), guinea pig polyclonal anti-CGRP antibody (1:300; Frontier Institute; GP-Af280), mouse monoclonal anti-neurofilament-M antibody (1:500; 1C8) , and mouse monoclonal anti-PV antibody (1:1000; Sigma-Aldrich; PARV-19, P3088), diluted in 0.1 M PBS with 0.01% Triton X-100 (PBST) containing 0.5% skim milk.

Techniques:

Journal: Science Advances

Article Title: Sensory-motor circuit is a therapeutic target for dystonia musculorum mice, a model of hereditary sensory and autonomic neuropathy 6

doi: 10.1126/sciadv.adj9335

Figure Lengend Snippet: ( A ) Histological analysis of neurodegeneration in each group at 3 weeks of age. In DRG of Dst Gt / Gt mice injected with AAV9-GFP, ATF3 and NF accumulation was abundantly observed, and there were few VGluT1-positive axon terminals around motor neurons in the AH of lumbar SC. In DRG of Dst Gt / Gt mice injected with AAV9-Cre, ATF3 and NF accumulation was scarcely observed, and VGluT1-positive axon terminals were abundant. PV-positive proprioceptive neurons were few in the DRG of Dst Gt / Gt mice injected with AAV9-GFP while normally observed in Dst Gt / Gt mice injected with AAV9-Cre. ( B ) Quantification of numbers of ATF3-positive cells [Ctrl ( n = 4 mice); Dst Gt / Gt ( n = 4); Dst Gt / Gt + AAV9-Cre ( n = 5)], NF-accumulating cells [Ctrl ( n = 3); Dst Gt / Gt ( n = 3); Dst Gt / Gt + AAV9-Cre ( n = 5)], PV-positive cells [Ctrl ( n = 4); Dst Gt / Gt ( n = 5); Dst Gt / Gt + AAV9-Cre ( n = 5)], and VGluT1-positive axon terminals [Ctrl ( n = 3); Dst Gt / Gt ( n = 3); Dst Gt / Gt + AAV9-Cre ( n = 5)]. ( C ) Quantification of electrophysiological data in mice at 2 to 3 weeks of age. H/M amplitude ratio and H-reflex latency in Dst Gt / Gt mice were rescued by injection with AAV9-Cre. [H/M amplitude ratio and H-reflex latency, Ctrl ( n = 8); Dst Gt / Gt ( n = 9); Dst Gt / Gt + AAV9-Cre ( n = 4); sample sizes are numbers of successful evocation of H-reflex; M response latency, n = 10 Ctrl; n = 16 Dst Gt / Gt ; n = 6 Dst Gt / Gt + AAV9-Cre; sample sizes are numbers of measurement of M response]. Scale bars, 50 μm (A). Data are presented as mean ± SE. P > 0.05 (ns), * P < 0.05, and *** P < 0.005, using ANOVA with Tukey’s test in (B) and (C).

Article Snippet: For IHC, deparaffinized sections were treated with microwave irradiation in 10 mM citric acid buffer (pH 6.0) for 5 min and incubated overnight at 4°C with the following primary antibodies: rabbit polyclonal anti-ATF3 antibody (1:2000; Santa Cruz Biotechnology; sc-188), rabbit polyclonal anti-VGluT1 antibody (1:1000; Frontier Institute, Hokkaido, Japan; Rb-Af500), goat polyclonal anti-VGluT1 antibody (1:1000; Frontier Institute; Go-Af310), goat polyclonal anti-ChAT antibody (1:500; Millipore; AB144P), guinea pig polyclonal anti-CGRP antibody (1:300; Frontier Institute; GP-Af280), mouse monoclonal anti-neurofilament-M antibody (1:500; 1C8) , and mouse monoclonal anti-PV antibody (1:1000; Sigma-Aldrich; PARV-19, P3088), diluted in 0.1 M PBS with 0.01% Triton X-100 (PBST) containing 0.5% skim milk.

Techniques: Injection